HPLC Instrument

 HPLC Instrument

1-Mobile-Phase Reservoirs

           One or more glass or stainless steel reservoirs

2-Pumping Systems

3-Sample Injection Systems:

              Syringe injection through a self-sealing elastomeric septum

4-Chromatographic Columns

    -Guard Columns

Ø  Remove impurities from solvent

Ø  Saturates mobile phase with liquid of stationary phase before the analytical column

              

-        Analytical Column

           Copper or stainless steel tubing straight, 15 to 150 cm in length; 2 to 3 mm packing - silica gel, alumina, ….

5-Column Thermostats

6-Detectors