Fluorescence Spectroscopy
(Molecular Emission)
Molecular fluorescence spectroscopy is an analytically important emission process in which molecules are excited by absorption of UV or visible radiation. Molecular fluorescence methods are widely used for qualitative and quantitative analysis.
A molecule in its ground state is stable.
Ø The absorption of UV or visible light raises the molecule to an excited high energy state.
Ø A molecule only lives in an excited state for a very limited time (10-9),and returns to the ground state by loss of the excess energy.
Quantitative Analysis
F = K C
F : emission intensity
K : constant
C : Concentration
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