Wednesday, August 24, 2011

Mechanisms of chromatography




Mechanisms of chromatography

n Adsorption
         A charge interaction occur between the active group of the sample and the sorbent S.F.

n Partition
   The separation depends upon the distribution of the compound of the mixture between the mobile phase and a liquid stationary phase according to partition coefficient value.

n Ion Exchange
                   Ion exchange contains labile ions that are capable of exchanging with ions of the same change in the sample.

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